Room 304, Chemistry Building
Professor Daniel Chiu
University of Washington
We have developed a method for isolating rare cells, such as circulating tumor cells (CTCs) and fetal cells, in blood based on positive selection, where cell-surface markers are labeled with fluorescent antibodies, ranked by aliquots, and sorted. We call this process ensemble-decision aliquot ranking (eDAR), because we perform the ranking by looking at an ensemble of cells within each aliquot. This presentation will describe our recent work using eDAR as a new platform for performing single-cell analysis and rare-cell diagnostics.
Daniel T. Chiu is the A. Bruce Montgomery Professor of Chemistry, Endowed Professor of Analytical Chemistry, and Professor of Bioengineering at the University of Washington, Seattle. He obtained a B.A. in Neurobiology and a B.S. in Chemistry from the University of California at Berkeley in 1993, then a Ph.D. in Chemistry from Stanford University in 1998. After completing postdoctoral research at Harvard University, he started in the Fall of 2000 at the University of Washington. He is currently a member of the Molecular Engineering and Sciences Center and the Neurobiology and Behaviour Program at the University of Washington, as well as a member of the Cancer Consortium at the Fred Hutchinson Cancer Research Center.